Naslov
A method of preparing frersh blood samples for Croatian haematology external quality assessment

Autori
Nazor A ; Flegar-Meštrić, Z ; Hećimović, A ; Šiftar, Z ; Hundrić Hašpl, Ž.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Biochemia Medica 2012 ; 22(3):267-402 ; A17-A204 / Šimundić, AM - Zagreb : Hrvatsko društvo za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM), 2012, A117-A117

Skup
2nd European Joint Congress of European Federation of Clinical Chemistry and Laboratory Medicine (EFCC) and European Union of Medical Specialists (UEMS)/ 7th Congress of Croatian Society of Medical Biochemistry and Laboratory Medicine (CSMBLM)

Mjesto i datum
Dubrovnik, Hrvatska, 10.10.2012. - 13.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
fresh blood samples; EQA

Sažetak
Background. Croatian EQA involves participation in the National External Quality Assurance Scheme organized under the auspices of the Croatian Society of Medical Biochemists by processing of unknown samples 3 times per annum. The suitability of home-made control material for complete blood count was evaluated. Materials and methods. The Croatian Institute for Transfusion Medicine prepared the homemade control material for complete blood count. A single portion of fresh blood is used given by voluntary donor having given consent to its intending use. A minor portion of the blood is tested for Hepatitis B and C as well as HIV and TP and was nonreactive for all these markers. After 30 minutes mixing in a way without making foam, 1, 5 ml K2EDTA molarity 1 mol/L is added and the mixing was continued next 2-5 minutes. The blood is distributed into suitable 7, 5 x 12 mm sterile tubes, capped and stored at +2 to +8 C. The samples were analyzed for homogeneity and stability accordiing to the ISO 13528 requirements. Results. The home made samples are delivered within 24 hours throughout Croatia in 200 medical biochemistry laboratories and are analyzed on 37 different types of analyzers from 11 different manufacturers. Assessment against consensus values and the percent allowed difference score is introduced for evaluation of obtained results. The high degree of inter-laboratory comparability is approved. Conclusion. The home made control material used in Croatian EQA scheme shows applicability to all haematological analyzers and provides a long term, retrospective assessment of laboratory performance and comparability of results on national level.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA