Naslov
Clenbuterol and salbutamol blood concentrations in treated animals

Autori
Pleadin, Jelka ; Durgo, Ksenija ; Vulić, Ana ; Perši, Nina ; Terzić, Svjetlana ; Mihaljević, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) / Želježić, Davor - Zagreb : Hrvatsko toksikološko društvo, 2012, 64-64

Skup
CROTOX

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
β-adrenergic agonist; clenbuterol; ELISA; guinea pigs; plasma; serum; salbutamol

Sažetak
The β-adrenergic agonist clenbuterol and salbutamol are pharmacologically active substances with anabolic effects, which bind to the specifi c β-adrenergic receptors found on cell membranes of the animal target tissues. The use of these substances as growth promoters for fattening purposes has been banned in the European Union. The aim of the present study was to determine clenbuterol and salbutamol blood concentrations, separately in the plasma and serum of animals after their exposure. The experimental group of guinea pigs (n=20) were treated with two dosages (0.25 mg kg-1 and 2.5 mg kg-1) of clenbuterol (n=10) and salbutamol (n=10) for 7 days, respectively, whereas the control group of animals (n=10) were left completely untreated. Validation of applied ELISA method showed acceptable recovery (R>70 %) and repeatability (CV<10 %) for all serum and plasma samples to which clenbuterol and salbutamol were added. This has proven the method to be applicable for quantitative determination of both analytes and matrices, preferably in plasma. Study results pointed to signifi cantly higher (about 14-fold) clenbuterol concentrations (1287±293) μg L-1 in comparison to salbutamol (95±20) μg L-1 in plasma, and generally higher concentrations of both analytes in plasma than in serum. Application of higher exposure dose resulted in about four-fold and three-fold higher clenbuterol and salbutamol concentrations, respectively, and pointed to a different rate of release of these two β-agonists.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija, Prehrambena tehnologija

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