Naslov
MKP-1 as a target for pharmacological manipulations in PC12 cell survival

Autori
Rumora, Lada ; Shaver, Alan ; Žanić Grubišić, Tihana ; Maysinger, Dusica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biochemia Medica / Suchanek, Ernest - Zagreb : Hrvatsko društvo za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM), 2000

Skup
6th Alps-Adria Congress of Clinical Chemistry and Laboratory Medicine

Mjesto i datum
Opatija, Hrvatska, 15.06.2000. - 17.06.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MPK-1; MAPK; monoperoxovanadium complex; PC12 cells

Sažetak
Dual specificity mitogen activated protein kinase phosphatase-1 (MKP-1) inactivates extracellular signal-regulated kinase (ERK), p38 and/or c-jun N-terminal protein kinase (JNK) by dephosphorylation via a negative feed-back loop. The aim of the present study was to assess the effectiveness, individually and in combination with the immunosuppressant agent FK506, of three structurally related monoperoxovanadium complexes on MKP-1 expression, on phosphorylation status of mitogen-activated protein kinase (MAPK), and on survival in PC12 cells. These compounds, known to be insulinomimetic agents and protein tyrosine phosphatase inhibitors, are cytotoxic to the cells, they activate JNK and down-regulate MPK-1. On the other hand, FK 506 has transient effect on ERK activation. However, when the agents are used in combination, MKP-1 expression is increased, and cell survival is enhanced. The concomitant alterations in the dynamic changes observed in signal intensities and duration of phospho-ERKs and phospho-JNKs signals suggest that in combination with monoperoxovanadium complexes, FK 506 enhances survival of PC12 cells by an induction of MKP-1 expression.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA