Naslov
Antifungal and aflatoxigenic activity of buthionine sulfoximine

Autori
Šarkanj, Bojan ; Juranić, Sanja ; Klapec, Tomislav ; Zec Zrinušić, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
5th Croatian Congress of Microbiology with International Participation Book of Abstracts / Černi, Silvija ; Šeruga Musić, Martina ; Škorić, Dijana - Zagreb : Hrvatsko mikrobiološko društvo, 2012, 92-92

ISBN
978-953-778-05-7

Skup
5th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Primošten, Hrvatska, 26.09.2012. - 30.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Aspergillus flavus; Glutathione S – transferase; buthionine sulfoximine; antiaflatoxigenic; antifungal

Sažetak
Antifungal and antiaflatoxigenic effects of buthionine sulfoximine (BSO) were investigated against Aspergillus flavus NRRL 3251 on glucose minimum salts (GMS) microbiological medium. Butathionine sulfoximine is known inhibitor of glutathione S-transferase (GST), and GST is suspected to have transporter function in aflatoxin biosynthesis. To prove role of GST in aflatoxin biosynthesis, specific inhibitor of GST (BSO) was added to GMS medium in concentrations which are allowed for human use: 10, 1, 0.1 and 0.01 ppm. Media were incubated for 48, 96 and 144 hours after which mycelia was filtrated and weighted. In microbiological medium aflatoxins (B1, B2, G1, G2) were determined using immunoaffinity cleanup followed by LC-MS/MS analysis. Mycelium was disrupted using ultrasonic probe and in intercellular content catalase activity and protein concentration were determined. During incubation period BSO showed high antiaflatoxigenic effect reducing total aflatoxin production from 362.14 ppb to 37.91 ppb at 1ppm BSO concentration. BSO also showed significant antifungal activity reducing mycelial wet weight form total 2.5 g after 144 hours of incubation to 0.40 g. In biochemical markers BSO showed reduction in protein biosynthesis, but catalase activity has increased over the growth period. Increased catalase activity can be explained by higher oxidative stress in A. flavus due to lack of GST activity, and this can also be caused by incomplete aflatoxin biosynthesis inhibition.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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