Pregled bibliografske jedinice broj: 59724
IMPAIRED INTERNODAL IONIC CONDUCTANCES IN MYELINATED AXONS OF PERIPHERAL NERVES FROM TRANSGENIC MICE WITH DIFFERENT NEUROFILAMENT EXPRESSION
IMPAIRED INTERNODAL IONIC CONDUCTANCES IN MYELINATED AXONS OF PERIPHERAL NERVES FROM TRANSGENIC MICE WITH DIFFERENT NEUROFILAMENT EXPRESSION // FIFTH IBRO WORLD CONGRESS OF NEUROSCIENCE : Abstracts
Jeruzalem, Izrael, 1999. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 59724 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
IMPAIRED INTERNODAL IONIC CONDUCTANCES IN MYELINATED AXONS OF PERIPHERAL NERVES FROM TRANSGENIC MICE WITH DIFFERENT NEUROFILAMENT EXPRESSION
Autori
Križ, Jasna ; Zhu, Q. ; Meyer, J. ; Simonić, Ante ; Julien, J.P. ; Padjen, Ante L.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
FIFTH IBRO WORLD CONGRESS OF NEUROSCIENCE : Abstracts
/ - , 1999
Skup
FIFTH IBRO WORLD CONGRESS OF NEUROSCIENCE
Mjesto i datum
Jeruzalem, Izrael, 11.07.1999. - 15.07.1999
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
myelinated axons; transgenic mice; neurofilaments
Sažetak
Neurofilaments (NFs) are the major cyoskeletal component in large myelinated axons of the peripheral nervous system, and are composed of three prolein subunits, NF-L, NF-M and NF-H, with molecular weight of approximately 70 kD, 145 kD and 200 kD, forming the rope like structures. NFs play a critical role in determining axonal caliber and consequently conduction velocity of large myelinated fibers. The aim of this study was to examine the electrophysiological properties of myelinated axons with altered morphology in transgenic mice with different NF-H expression (NF-H knockout mice and mice overexpressing human NF-H, line 200 mice). Single axons membrane properties were measured on isolated sciatic nerves in vitro using intracellular standard current-clamp technique. Transgenic animals showed several functional deficits in physiological properties. In NF-H knockouts voltage responses revealed increased steady state input resistance (dRss) during depolarization (12.1 ą 1.0 Mohm control vs. 15.5 ą 1.0 Mohm ) and increased peak resistance during evoked hyperpolarization (hRp) (33.5 ą 2.4 Mohm, control vs. 42.6 ą 3.9 Mohm) (control n=28, NF-H-/- n=14). Axons from line 200 transgenic mice showed significant increase in peak (dRp) (14.2 ą 0.95 Mohm, control vs. 23.11 ą 1.7 Mohm) and dRss (12.07 ą 0.82 Mohm vs. 20.44 ą 1.12 Mohm) (control n=28, line 200 n=15) during depolarization associated with impaired inward rectification . Our studies demonstrate that targeted NF-H disruption or human NF-H hyperexpression in transgenic mice affect functional characteristics of large myelinated fibers ( ion channel defects ), suggesting a complex structure/function relationship distinct from previously established structural role of NF.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
