Naslov
In vitro antitumor effect of domestic Apis mellifera bee venom and melittin

Autori
Marijana Jukić, Katarina Mišković, Drago Bešlo, Ljubica Glavaš-Obrovac

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
International Scientific and Professional Conference 14th Ruzicka Days „Today Science – Tomorrow industry“ / - , 2012, -----

Skup
International Scientific and Professional Conference 14th Ruzicka Days „Today Science – Tomorrow industry“

Mjesto i datum
Vukovar, Hrvatska, 13.09.2012. - 15.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
pčelinji otrov ; melitin ; protutumorski učinak ; HeLa
(bee venom ; melittin ; antitumor activity ; HeLa)

Sažetak
Bee venom therapy has been used as a traditional medicine to treat a variety of illness, such as arthritis, rheumatism, back pain, tumours, and skin diseases. To test antitumor potential of domestic Apis mellifera bee venom (BV) we used selected cell lines, tumour colon adenocarcinoma (CaCo-2), cervix adenocarcinoma (HeLa), Burkitt lymphoma (RAJI), chronic myeloid leukaemia in blasted crisis (K562) of human origin and Madine-Darby canine kidney (MDCK) cells. Final concentration range of BV was 0.001 to 1 mg/mL. As a standard compound was used melittin in a concentration range of 0.001 to 0.1 mg/mL. The cytotoxic effect of BV and melittin were evaluated after 72 hours of incubation by the MTT assay. Obtained results showed that leukaemia cells were more sensitive to BV and melittin. The 50% survival of treated tumor adherent cell (IC50) was achieved by application of 0.1 mg/mL of BV and melittin. As expected, the most resistant to BV and melittin was normal MDCK cells. BV as a natural remedy, through the activity of lytic peptides mellitin, can be useful as a new targeted therapy in anticancer treatment.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

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