Naslov
Isolation methods for mycotoxins - The good, the bad and the ugly

Autori
Šarkanj, Bojan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) u Arhiv za higijenu rada i toksikologiju, Vol. 63, Supplement 2 / Željezić, Davor - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2012, 42-42

Skup
4th Croatian Congress of Toxicology (CROTOX 2012)

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
detection of mycotoxins in food ; extraction methods ; sample preparation

Sažetak
In most cases, isolation methods for mycotoxins are composed of several processes: sample preparation, mycotoxin extraction, purifi cation, and concentration. Due to low concentration of mycotoxins in food, analysis is impossible without sample pre-treatment, excluding enzyme linked immunosorbent assay (ELISA) and highly sensitive LC-MS/MS devices, following a simple dilute-and- shoot method. Sample preparation is one of the most important steps, which must meet all analytical criteria and legislation requirements. There are different strategies used to extract mycotoxins dependent on the sample matrix and targeted mycotoxin physicochemical properties. Since raw extracts can contain a lot of impurities from sample matrix, they can be removed using various approaches. The most common are: solid phase extraction (SPE) in the form of immunoaffi nity columns (IAC), ion exchange columns (IEC) fi lled with adsorbents or normal phase (NP) reverse phase (RP) fi lled solid phase columns. Newer SPE techniques comprise the usage of aptamers (short DNA or RNA strains which are able to detect target molecules) and molecular imprinted polymers (MIP). There is also a possibility of liquid/liquid extraction (LLE), which is used mainly for complex matrices due to some diffi culties such as the use of a high amount of organic and chlorinated solvents, formation of emulsions, and co- extraction of impurities. There is no simple answer as to which isolation method is the best for your mycxotoxin(s) of interest - it depends on its physicochemical properties and used detection method, but generally they can be classifi ed as “the good, the bad, and the ugly”.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA