Naslov
Effect of Angiotensin II on Inflammation Pathways in Human Primary Bone Cell Cultures in Otosclerosis

Autori
Rudić, Milan ; Nguyen, C. ; Nguyen, Y. ; Milković, Lidija ; Žarković, Neven ; Sterkers, O. ; Ferrary, E. ; Grayeli, A.B.

Izvornik
Audiology and neuro-otology (1420-3030) 17 (2012), 3; 169-178

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
bone dystrophy; Angiotensin II; hearing loss; inflammation; bone remodeling

Sažetak
The aim of this study was to assess the expression and production of inflammation mediators in basal condition and after angiotensin II (AngII) in otosclerosis. Human stapedial cell cultures (6 otosclerosis and 6 controls) were incubated with AngII (10–7M, 24 h) or vehicle. Cytokines and their mRNA expression were assessed by antibody and cDNA arrays. In basal conditions, otosclerotic cultures produced higher amounts of interleukin (IL)-1β and interferon-inducible protein 10, and smaller amounts of tissue inhibitor of metalloproteinase 2. AngII promoted inflammation by increasing interferon γ and IL-10, and by decreasing macrophage inflammatory protein 1α and soluble tumor necrosis factor receptor II. Otosclerotic cultures produced higher proinflammatory cytokines in basal condition. AngII appeared to promote inflammation via these mediators in otosclerosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA