Naslov
Differentiation and activation of macrophages: a galectin-3 perspective

Autori
Novak, Ruđer ; Dabelić, Sanja ; Dumić, Jerka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEBS Journal ; Special Issue: 22nd IUBMB & 37th FEBS Congress, Seville, Spain, September 4-9, 2012 / - Sevilla : John Wiley & Sons, 2012

Skup
22nd IUBMB & 37th FEBS Congress

Mjesto i datum
Sevilla, Španjolska, 04.09.2012. - 09.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
galectin-3; macrophages

Sažetak
Galectin-3 (Gal-3), a β-galactoside lectin, is mainly considered to advance inflammation: it triggers/promotes monocyte respiratory burst, acts as a monocyte (Mc)/macrophage (Mf) chemoattractant and promotes the survival of inflammatory cells. Depending on environmental cues, Mc/Mf exhibit diverse biological characteristics, but two main subtypes, classically (M1) and alternatively (M2) differentiated and activated Mf have been recognized. This study explores the expression of Gal-3 in the physiology of these cells. Monocytes from healthy blood donors were differentiated to M1 or M2 cells using macrophage colony-stimulating factor (M-CSF) or granulocyte- macrophage colony- stimulating factor (GM-CSF), respectively. Macrophages were then activated classically by IFN-γ and LPS, or alternatively, using IL-4/IL-10 to generate M2a/c cells. Gene and protein expression levels of intra- and extracellular Gal- 3 were investigated by qRT-PCR, Western-blot, flow cytometry, imunoprecipitation and ELISA, while surface Gal-3 receptor expression was analyzed by flow cytometry. Differentiation of Mc and classical/alternative Mf activation was followed by marked changes of Gal-3 expression and proteolitic cleavage. Furthermore, its expression and secretion were tightly regulated and significantly differed among distinctly differentiated and activated Mf. Interestingly, considerable differences in Gal-3 expression profiles were observed among the same Mf subtypes, obtained from different donors. Upon activation by IFN-γ and LPS, M1 (but not M2) macrophages diverged into distinct populations with respect to membrane Gal-3 expression. Human Mc had a high amount of free Gal-3 receptors, while on activated Mf the receptors were fully saturated. Specific expression and secretion patterns of Gal- 3 in differentiated and activated Mf contribute to better understanding of its role in these cells, and provide an important insight into macrophage biological characteristics.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA