Naslov
The potential use of apathogenic Trypanosomatids as alternative source of antigen for serological screening of leishmaniasis in dogs.

Autori
Martinković Franjo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Multicolloquium Of Parasitology XI, Program and Abstract Book, EMOP XI, Cluj-Napoca, Romania, 25 – 29 July 2012 / - , 2012, 414-414

Skup
EMOP XI, EUROPEAN MULTICOLLOQUIUM OF PARASITOLOGY XI, Cluj-Napoca, Romania, 25 – 29 July 2012

Mjesto i datum
Cluj-Napoca, Rumunjska, 25.07.2012. - 29.07.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Leishmania infantum; crossreactivity; apathogenic trypanosomatids; indirect immunofluorescence

Sažetak
Leishmaniasis is an anthropozoonotic disease distributed in many areas of the world, caused by many members of Leishmania genus. In Europe it is caused with two endemic species, Leishmania infantum and Leishmania tropica. They belong to the genus Leishmania, family Trypanosomatidae, a family of monoxenous parasites of insects as genera Leptomonas, Crithidia, Blastocrithidia, Herpetomonas, heteroxenous parasites of insects and plants as genus Phytomonas and heteroxenous parasites of insect and vertebrates as genera Trypanosoma and Endotrypanum. Two genera, Leptomonas and Phytomonas, are apathogenic for vertebrates. In their original hosts and in in vitro culture, they occur in the same promastigote form as Leishmania parasites in its insect host (sand flies) and in in vitro culture. Sources of antigen for serological tests in routine diagnostics of leishmaniasis are usually in vitro cultivated Leishmania promastigotes, which are known to be infective for laboratory workers under certain conditions. Because of its infectivity for laboratory workers, its same form of growth in in vitro cultures as apathogenic genera and its belonging to the same family with apathogenic trypanosomatids, the aim of this study was to define the potential use of in vitro cultivated harmless promastigote forms of Leptomonas seymouri and Phytomonas serpens as an alternative source of antigen in the routine animal serology. Three hundred dog sera were tested with indirect immunofluorescence assay and obtained results compared with the ordinary L. infantum promastigote antigen. The crossreactivity level was the same when compared with the ordinary Leishmania promastigote antigen. Obtained results point to the possibility of using harmless promastigote antigen of L. seymouri and P. serpens as a alternative source of antigen for the large scale serological screening of leishmaniasis in dogs.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA