Naslov
Improved sensitization of CD8+ T cell response after infection with MCMV expressing NKG2D ligand

Autori
Tršan, Tihana ; Slavuljica, Irena ; Busche, Andreas ; Babić Čač, Marina, ; Golemac, Mijo ; Mihelčić, Mirna ; Abram, Maja ; Krmpotić, Astrid ; Messerle, Martin ; Jonjić, Stipan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2011 Annual Meeting of the Croatian Immunological Society / - , 2011

Skup
Godišnji skup Hrvatskog imunološkog društva, Rabac 2011.

Mjesto i datum
Rabac, Hrvatska, 07.10.2011. - 09.10.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Cytomegalovirus; NKG2D; NKG2D ligands; CMV vaccine vector; CD8 T cell vaccine

Sažetak
We have developed a recombinant mouse cytomegalovirus expressing high affinity NKG2D ligand Rae-1γ (RAE-1γ MCMV). NKG2D is a potent activating receptor expressed by cells of both innate and adaptive immunity. Its importance in CMV immunosurveillance is indicated by the elaborative viral evasion mechanisms evolved to avoid NKG2D. Expression of Rae-1γ by MCMV resulted in profound virus attenuation in vivo. Infection was efficiently controlled by adult and immunologically immature neonatal host as well as immunodeficient hosts. RAE-1γ MCMV was also highly attenuated in naturally more resistant C57BL/6 mice and induced even stronger virus specific CD8+ T cell response upon higher viral load. At early days post infection RAE-1γ MCMV decreased systemic IFNα level, preserved conventional dendritic cells and promoted effector CD8+ T cell response. Despite tight immune control, RAE-1γMCMV infection elicited strong, long-lasting protective immunity. These results prompted us to test vector capacity of Rae-1γ MCMV. Our results indicate that a recombinant virus encoding ligand for the NKG2D receptor might provide a new, powerful approach for development of a safe immunogenic CMV vaccine as well as CMV-based vaccine vector.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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