Naslov
Production of (S)-2-hydroxypropiophenone catalyzed by benzoylformate decarboxylase in different reactor types

Autori
Husanović, Dina ; Valinger, Davor ; Vrsalović Presečki, Ana ; Kurtanjek, Želimir ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Applied Biocatalysis, 8th meeting of students and university professors, Book of abstracts / Vrsalović Presečki, Ana ; Vasić-Rački, Đurđa - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2012, 16-16

ISBN
978-953-6470-59-4

Skup
Applied Biocatalysis, 8th meeting of students and university professors

Mjesto i datum
Zagreb, Hrvatska, 19.09.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
benzoylformate decarboxylase; (S)-2-hydroxypropiphenone; process modelling; reaction kinetics

Sažetak
Hydroxy ketones are of great interest in preparative organic chemistry as substrates for a vast number of applications [1]. The use of enzyme benzoylformate decarboxylase allows the production of enantiopure 2-hydroxy ketones in high yield starting from widely available aldehydes [2]. In this study the synthesis of (S)-2- hydroxypropiphenone (S-(2)-HPP) from benzaldehyde and acetaldehyde was catalyzed by benzoylformate decarboxylase from Pseudomonas putida [3]. The reaction was carried out in different types of the reactors: repetitive batch, continuously operated membrane reactor and microreactor. Reaction kinetics was described by double substrate Michaelis Menten equation with substrate (acetaldehyde) inhibition. Acetaldehyde which is a substrate in the reaction caused a significant enzyme deactivation. This deactivation was described by biexponential model. Mathematical model of the process was proposed and it was validated by the experimental results gathered in the batch and continuously operated membrane reactor. All experiments were performed with the 350 % excess of the acetaldehyde. In the batch reactor 100 % benzaldehyde conversion was achieved. This wasn’t a case in a continuously operated membrane reactor. The reason was relatively high enzyme deactivation rate. Four different types of microreactor were used for the synthesis of S-(2)-HPP in order to examine the impact of microchannel geometry and mixing on the conversion of benzaldehye. In all types of microreactor the complete benzaldehyde conversion was accomplished for the retention time of ten minutes [1] Hildebrand F., Kuhl S., Pohl M., Vasić- Rački Đ., Muller M., Wandrey C., Lutz S., Biotechnol Bioeng, 2007, 96:835-843 [2] Pohl M., Lingen B., Muller M., Chem Eur J, 2002, 8:5288-5295 [3] Pepper S., Kara S., Long W.S., Liese A., Niemeyer B., Bioprocess Biosyst Eng, 2011, 34:671- 680

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA