Naslov
Cultivation of Pseudomonas putida in different configurations of bioreactors

Autori
Pandurić, Nikola ; Džebrić, Bojan ; Kozina, Irena ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka: XIV. Ružičkini dani "Danas znanost - sutra industrija" / Jukić, Ante - Osijek : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2012, 18-18

ISBN
978-953-6894-46-8

Skup
XIV. Ružičkini dani "DANAS ZNANOST - SUTRA INDUSTRIJA"

Mjesto i datum
Vukovar, Hrvatska, 13.09.2012. - 15.09.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Pseudomonas putida; bioreactor; growth; biomass

Sažetak
Pseudomonas putida is an industrially very interesting microorganism with an ability to degrade numerous toxic compounds. Because of it, this gram-negative rod shaped bacteria is a big potential for long term solutions of environmental problems caused by toxic compounds as a result of their use in agriculture and other businesses. It is very adaptable to different growth conditions and characterized by a big metabolic enzyme portfolio. In this work, Pseudomonas putida strains KT2440 and VLB120∆C were cultivated in a batch, fed-batch and continuous reactor. During batch cultivation, the influence of two different growth media, M9 and M12, for microorganism cultivation were investigated by experiments with both strains. Better results were achieved in experiments with starting concentration of glucose γ = 15 gdm3 by cultivating bacteria on M12. For strain VLB120∆C highest specific growth rate of  = 0.837 h-1 was recorded with final maximum biomass concentration of cX = 11.58 g dm-3. Same media was used in fed- batch experiments where highest growth rate of  = 0.599 h-1 and maximum biomass concentration of cX = 11.98 g dm-3 were achieved in experiment with specific glucose feed rate of 9.5 g h-1 dm-3. In experiments performed in continuous reactor for different retention times, M12 growth media with 15 g dm-3 glucose was used as inlet stream. The experiment was carried out for 5 days and maximum biomass concentration of cX = 7.50 g dm-3 was achieved for retention time of  = 20 h. Based on current research efforts, batch reactor is best reactor configuration for Pseudomonas putida cultivation if reactors were evaluated by highest specific growth rate achieved.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

Napomena
Ovaj se tekst temelji na radu u sklopu projekta Pseudomonas 2.0:industrial biocatalysis using living cells, projekt br. EIB.10.041

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