Naslov
Effects of antifoaming agents on the cultivation of Pseudomonas putida

Autori
Folo, Ana ; Pandurić, Nikola ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts "Applied Biocatalysis" 8th meeting of students and university professors / Vrsalović Presečki, Ana ; Vasić-Rački, Đurđa - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2012, 19-19

ISBN
978-953-6470-59-4

Skup
"Applied Biocatalyis" 8th meeting of students and university professors

Mjesto i datum
Zagreb, Hrvatska, 19.09.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Pseudomonas putida; biomass growth; foaming; antifoam agents

Sažetak
Pseudomonas putida is a gram-negative, rod shaped bacterium which has the capability to degrade numerous toxic organic compounds deconstructing aliphatic or aromatic hydrocarbon chains. These bacteria have a large number of potential applications and can be used in various biotechnological processes including bioremediation of contaminated areas, quality improvement of fossil fuels, biocatalytic production of fine chemicals, production of bioplastic and as agents of plant growth promotion and plant pest control [1]. This study was conducted to examine the impact and effectiveness of 8 different antifoam agents on the cultivation of P. putida. The appearance of the foam is very common, especially in aerobic fermentations and is due to the presence of protein at the gas-liquid interface. Foaming can lead to reduced yields since bubbles can damage proteins [2] and can also result in loss of sterility if foam escapes [3]. Addition of chemical antifoam agents is a common method for preventing the formation of foam. Small quantities of antifoam are needed and it has low contribution to production costs. It is reliable at extreme foaming conditions and easy to handle. Tests were performed in a glass column (500 mL) with 150 mL of foaming medium (P.putida was grown in synthetic medium M12). Medium was continuously stirred by magnetic stirrer and airflow was supplied at the base of the column with a porous diffuser. Foam height and oxygen concentration were recorded before and after the addition of antifoam agents. The success of antifoam agents was assessed by the rate of foam destruction and the amount of consumed antifoam. The formation of new foam indicated unreliable antifoam agent. Agents had almost no effect on the concentration of oxygen that remained more-less constant. Antifoam agent ASP-664 proved to be the most successful in suppressing foam formation.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

Napomena
Ovaj se tekst temelji na radu u sklopu projekta Pseudomonas 2.0:industrial biocatalysis using living cells, projekt br. EIB.10.041

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