Naslov
Aldol addition catalyzed by two different D- fructose-6-phosphate aldolases

Autori
Smailbegović, Emina ; Sudar, Martina ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Applied Biocatalysis, 8th meeting of students and university professors / Vrsalović Presečki, A. ; Vasić-Rački, Đ. - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2012, 9-9

ISBN
978-953-6470-59-4

Skup
Applied Biocatalysis, 8th meeting of students and university professors

Mjesto i datum
Zagreb, Hrvatska, 19.09.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
aldol addition; D-fructose-6-phosphate aldolase

Sažetak
Aldolases belong to a group of enzymes called lyases and they catalyze carbon-carbon bond formation [1]. In this work aldol addition of dihydroxyacetone (DHA) to N-Cbz-3-aminopropanal (aldehyde) was studied (Scheme 1). The aldol product of the reaction studied in this work is used as precursor in the production of fagomine, a naturally occurring iminosugar with remarkable biological properties [2]. Scheme 1. The reaction scheme The reaction investigated in this work was catalyzed by D-fructose-6-phosphate aldolase from genetically modified E. coli. Two different aldolases were investigated, FSA A129S and FSA A129S/A165G. In order to determine the best conditions for this reaction, different types of reactors were used. The reaction of aldol adition was carried out in a batch reactor, in enzyme membrane reactor and in a microreactor.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA