Naslov
Effects of abscisic acid and carbohydrates on the maturation of Picea omorika (Panč.) Purk. somatic embryos

Autori
Tramišak-Milaković, Tamara ; Mihaljević, Snježana ; Jelaska, Sibila

Izvornik
Acta botanica Croatica (0365-0588) 58 (1999), 1; 87-97

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Picea omorika ; omorika spruce ; somatic embryogenesis ; abscisic acid ; embryo maturation ; carbohydrates

Sažetak
In eight embryogenic tissue lines of omorika spruce the roles of carbohydrates and abscisic acid on embryo maturation were examined. Sucrose, glucose and maltose (3 % and 6 %) with abscisic acid (20, 40, 60 uM) were screened for optimal combination with respect to embryo maturation. The B lines, which expressed a brown tissue phenotype, achieved superior embryo maturation on 3 % glucose and 40 uM abscisic acid. The W lines with a white and translucent tissue phenotype showed the best maturation capacity on 3 % sucrose and 3 % maltose with 20 or 40 uM ABA. Sixty uM ABA inhibited embryo maturation. Media containing 6 % of either sucrose, glucose and maltose produced fewer mature embryos compared to media with 3 % of the same sugars. The W lines had almost three times lower maturation capacity in relation to the B lines.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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