Naslov
NAD+ coenzyme regeneration in microeractor using permeabilized whole cells of baker's yeast

Autori
Faletar, Petra ; Šalić, Anita ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts "Applied Biocatalysis" 8th meeting of students and university professors / Vrsalović Presečki, A ; Vasić-Rački, Đ - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2012, 10-10

ISBN
978-953-6470-59-4

Skup
"Applied Biocatalyis" 8th meeting of students and university professors

Mjesto i datum
Zagreb, Hrvatska, 19.09.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
coenzyme regeneration; NAD+; ADH; yeast; microreactor

Sažetak
NAD(P)-dependent oxidoreductases represent a great interest in the field of biotechnology and biotransformation. Although they have many advantages, the biggest drawback and limitation of oxidoreductase usage is the price of the coenzymes. Coenzymes are essential for the enzyme’s functionality, since they should be added in a stoichiometric amount and may not be replaced by more economical synthetic products. In order to solve this problem, many in-situ methods have been studied and developed for regeneration of coenzymes. Unfortunately, although results indicate that those methods are suitable for regeneration procedure, most of the processes need additional optimization to make them more sustainable. As an alternative, microreactor technology, due to many advantages, could be used as a new technique for coenzyme regeneration processes. In this study regeneration of coenzyme NAD+ was carried out by acetaldehyde reduction to ethanol using enzyme alcohol dehydrogenase (ADH). Permeabilized whole cells of baker's yeast (Saccharomyces cerevisiae) were used as the source of the enzyme ADH and conversion of approximately X ≈ 70 % for the residence time lower than 50 s was achieved. Based on experimental results, by measuring dependence of the reaction rate on the concentration of all components of the reaction system, kinetics of coenzyme regeneration was estimated and the mathematical model of the process was developed. Validation of developed mathematical model of the process was carried out on a series of independent experiments.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA