Naslov
Hexanol oxidation in microreactor by immobilized cells

Autori
Pindrić, Katarina ; Šalić, Anita ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts "Applied Biocatalysis" 8th meeting of students and university professors / Vrsalović Presečki, A ; Vasić-Rački, Đ - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2012, 12-12

ISBN
978-953-6470-59-4

Skup
"Applied Biocatalyis" 8th meeting of students and university professors

Mjesto i datum
Zagreb, Hrvatska, 19.09.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
baker's yeast; ADH; hexanal; microreactor; immobilization; permeabilization

Sažetak
Although hexanal has number of applications in food and cosmetic industry because of his organoleptic characteristics, problems like low yields, formation of unvanted by-products and large quantities of waste in its traditional producing methods are the reasons for developing new hexanal's production methods. Application of biocatalysis-based transformation in microreactors as alternative to classical synthesis processes is being investigated. Microreactors have many advantages over classical systems, such as well defined reaction times, ability to automate the process, more predictable numbering-up instead of classical scale-up and high surface to volume ratio. Because conditions in microreactors can be precisely controlled, the quality of the product can also be improved. In this work, biocatalytic oxidation of hexanol to hexanal using whole baker's yeast cells was performed. Baker’s yeast has many advantages over conventional biocatalysts such as low price, stability and constancy in different external conditions. Prior to biotransformation cells were immobilized on a glass microchannel surface in a tubular microreactor (V = 6 mm3) using three different methods of covalent immobilization. The method of immobilization that was evaluated as the best, was then used in further processes of hexanal oxidation where maximal conversion od 8 % is achieved. The kinetic model for hexanal production catalyzed by baker's yeast cells in microreactor was described and the mathematical model was developed. The parametric analysis of estimated kinetic parameters has been conducted. Validation of developed mathematical model of the process was carried out on a series of independent experiments.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA