Naslov
Catechol biotransformation in a microreactor

Autori
Đukić, Irena ; Jurinjak Tušek, Ana ; Kurtanjek, Želimir ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts "Applied Biocatalysis" 8th meeting of students and university professors / Vrsalović Presečki, A ; Vasić-Rački, Đ - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2012, 14-14

ISBN
978-953-6470-59-4

Skup
"Applied Biocatalysis" 8th meeting of students and university professors

Mjesto i datum
Zagreb, Hrvatska, 19.09.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
catechol; biotransformation; microreactor

Sažetak
Laccases are monomeric, dimeric or tetrameric glycoproteins with four copper atoms (belonging to three types: 1, 2 or 3) per monomer located at the catalytic site. Laccase use molecular oxygen to oxidize a variety of aromatic and non-aromatic hydrogen donors (e.g. phenols, polyphenols, anilines) via a mechanism involving radicals. Laccase uses oxygen as the electron acceptor to remove protons from the phenolic hydroxyl groups. This reaction gives rise to radicals that can spontaneously rearrange, which can lead to fission of C-C or C-O bonds of the alkyl side chains, or to cleavage of aromatic rings. Due to that laccase have an application for industrial and/or bioremediation purpose. In this work catechol (1, 2-dihydroxybenzene) biotransformation using laccase form Trametes versicolor was performed in macro- and microreactor. For experiments performed in macroscale (batch reactor V = 50 cm3 without continuous aeration) maximum conversion achieved was X = 21.07 % after 1200 s. Three reactors were used for biotransformation on micro-scale: (i) microreactor with internal volume of 2 µL with two inflows (“Y” shaped) and one outflow equipped with micromixers (specific surface roughness of 1 µm), (ii) microreactor with internal volume of 6 µL with two “Y” shaped inflows and outflows without surface roughness and (iii) tubular PTFE reactor with internal volume of 235 µL. For the same initial conditions as in the batch experiment maximum conversion of X = 16.73 % was obtained in microreactor equipped with micromixers for residence time of only 24 s.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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