Naslov
Separation of proteins from human plasma by sample displacement chromatography in hydrophobic interaction mode

Autori
Josić, Đuro ; Breen, Lucas ; Clifton, James ; Srajer Gajdošik, Martina ; Gašo-Sokač, Dajana ; Ručević, Marijana ; Müller, Egbert

Izvornik
Electrophoresis (0173-0835) 33 (2012), 12; 1842-1849

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
human plasma; hydrophobic interaction; sample displacement chromatography; therapeutic proteins

Sažetak
Sample displacement chromatography (SDC) in reversed-phase and ion-exchange modes was introduced approximately 20 years ago. Thismethod was first used for the preparative purification of peptides and proteins. Recently, SDC in ion-exchange mode was also successfully used for enrichment of low-abundance proteins from human plasma. In this paper, the use of SDC for the separation of plasma proteins in hydrophobic interaction mode is demonstrated. By use of two or more columns coupled in series during sample application, and subsequent elution of detached columns in parallel, additional separation of bound proteins was achieved. Further low-abundance, physiologically active proteins could be highly enriched and detected by ESI-MS/MS

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA