Pregled bibliografske jedinice broj: 590512
Reprogramming the EMT phenotype with the microRNA- 200 family in ovarian cancers
Reprogramming the EMT phenotype with the microRNA- 200 family in ovarian cancers // 2011 Oncology-Hematology Annual Research Retreat
Pacific Grove (CA), Sjedinjene Američke Države, 2011. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 590512 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Reprogramming the EMT phenotype with the microRNA- 200 family in ovarian cancers
Autori
Brozovic, Anamaria ; Moisan, Francois ; Francisco, Brian ; Wang, C. Yan ; Sikic, I. Branimir
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
2011 Oncology-Hematology Annual Research Retreat
/ - , 2011
Skup
2011 Oncology-Hematology Annual Research Retreat
Mjesto i datum
Pacific Grove (CA), Sjedinjene Američke Države, 19.10.2011. - 21.10.2011
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
miRNA-200; ovarian cancer; drug resistance; paclitaxel; cisplatin
Sažetak
Our laboratory has developed several models of paclitaxel-resistant ovarian cancer cell lines characterized with non-MDR1 mechanisms of resistance by selection of wild-type OVCAR-3, ES-2 and MES-OV cells with increasing doses of paclitaxel and the inhibitor of P-glycoprotein, PSC-833. The resulting variants (OVCAR-3/TP, ES- 2/TP and MES-OV/TP) are showing up-regulation of epithelial-mesenchymal transition (EMT) genes ; vimentin, fibronectin, MMP2 and MMP9. Cell migration was markedly increased in resistant variants, but the growth rates of the variants in vitro were slower than parental cells. In contrast, the growth rate of the EMT variant OVCAR-3/TP in athymic mice was markedly higher than tumors from parental cells in both subcutaneous and intraperitoneal xenografts. All three resistant cell variants are characterized with down-regulation of microRNA-200 family members (200a, 200b, 200c, 141, 429). The goal of this project is to investigate the role of the microRNA-200 family in the regulation of EMT in OVCAR-3 cells and their variants, and its possible contribution to drug resistance and enhanced tumorigenesis in vitro and in vivo. The role of the miRNA-200 family will be investigated using two approaches: (1) up-regulation of miRNA-200s by infecting the paclitaxel-resistant OVCAR-3/TP cell line with lentiviral particles carrying the specific genomic sequences of each family member and a GFP marker gene ; and (2) the paclitaxel- sensitive variant OVCAR-3 will be infected with lentiviral particles carrying anti-miRNA constructs. Stable cell clones with up- or down- regulation of miRNA-200s will be sorted by GFP fluorescence. Downstream targets of miRNA-200s will be investigated as well as sensitivity to paclitaxel and cisplatin.
Izvorni jezik
Engleski
