Naslov
Impact of adhesive tapes and extraction methods in the determination of natural moisturizing factors by high performance liquid chromatography

Autori
Đapić I, Kezic S, Kammeyer A, Jakasa I

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
13th International Chromatography School / Š. Ukić, T. Bolanča - Zagreb, 2012, 46-46

ISBN
978-953-6470-58-7

Skup
13th International Chromatography School

Mjesto i datum
Zagreb, Hrvatska, 18.06.2012. - 19.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
natural moisturizing factor; tape stripping; stratum corneum; atopic dermatitis

Sažetak
Stratum corneum (SC) is the outermost part of the skin and has important role in body protection against penetration of chemicals and pathogens, UV radiation and keeps safe the water balance in and out of the body. Filaggrin (filament-aggregating protein) plays a major role in SC structural integrity and is proteoliticaly degraded into a pool of free amino acids and their metabolites (histidine, trans- and cis- urocanic acid, 2-pyrrolidone-5-carboxylic acid and tyrosine, ) known as natural moisturizing factors (NMFs). NMF constituents are hygroscopic and responsible for maintaining hydration of the SC, pH of the skin, bacterial defense etc. Atopic dermatitis, a common skin inflammatory disease is associated with reduced level of NMFs and thus determination of NMFs in SC is of great interest. Tape stripping is a simple and non-invasive method for sampling of the SC in vivo. In the present work we evaluated the performance of two different adhesive tapes to sample SC. Furthermore, we investigated two different methods for extraction of NMF components from the tape strips. We investigated two commercially available tape strips: D-Squame and Corneofix. Extractions of NMFs from these tape strips have been performed by using ammonia and potassium hydroxyde. NMFs recovered from the tape strips were analyzed by reversed phase high performance liquid chromatography. To normalize for the amount of the SC harvested by the tape, we determined the amount of proteins on the tape by measuring 1) optical density and 2) colorimetric method (Pierce assay). This investigation provided more insight in the factors that influence performance of the method used for the determination of NMFs in the SC.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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