Naslov
Age and gender dependent biochemical changes in central nervous system of follitropin receptor knockout mice

Autori
Rumora, Lada ; Lovrić, Jasmina ; Sairam, Ram M. ; Maysinger, Dusica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biochemia Medica / - Zagreb : Medicinska naklada, 2006, S157-S157

Skup
5. hrvatski kongres medicinskih biokemičara s međunarodnim sudjelovanjem

Mjesto i datum
Poreč, Hrvatska, 18.10.2006. - 22.10.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Hsp; MAPK; FORKO miševi
(Hsp; MAPK; FORKO mice)

Sažetak
Aging is accompanied by enhanced plasma concentrations of gonadotropins and sex hormone imbalance, which could increase the risk for neurodegenerative disorders. We assessed the status of heat shock proteins (Hsps) and mitogen-activated protein kinases (MAPKs) in hippocampus and cortex of follitropin receptor knockout (FORKO) mice with enhanced LH and FSH together with estradiol and testosterone imbalance, as a consequence of FSH-R deletion. MAPK expression was unchanged in the brain structures examined, regardless of genotype, age or gender. However, Hsp expression and MAPK activation were age and gender dependent. In the hippocampus, expression of Hsp70 was reduced in 20 months old FORKO males and both Hsp70 and Hsp25 were down-regulated in age-matched females. In addition, female FORKO mice exhibited enhanced P-ERK already at 3 months of age while these changes become significant only in 20 months old male knockouts. In the cortex, the most distinguished changes were observed in older animals (20 months of age). Hsp25 was slightly decreased in males while the expression of Hsp70 and Hsp25 was significantly lower in females. The signal intensity of P-ERK was strong in both genders. Functional characteristics of MAPKs are dependent on their subcellular localization. In the hippocampus of 12 months old FORKO females translocation of P-ERK to the nucleus decreased and more cytosolic P-ERK was found then in the age-matched wild-type animals. On the other hand, nuclear P-JNK was significantly reduced whereas it was markedly enhanced in the mitochondria of the cortex of 20 months old FORKO males. In summary, enhanced gonadotropins alone or in combination with sex hormone imbalance in aging lead to biochemical changes in CNS in terms of disturbed Hsps expression, activation and subcellular distribution of MAPKs, and those changes could present a risk factor for neurodegeneration in menopause and late andropause.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA