Naslov
Disturbed Hsps expression and MAPKs activation in the hippocampus and the cortex of FORKO mice

Autori
Rumora, Lada ; Lovrić, Jasmina ; Sairam, Ram M. ; Maysinger, Dusica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Kongres hrvatskog društva za biokemiju i molekularnu biologiju prigodom 30. obljetnice osnutka uz međunarodno sudjelovanje / Kovarik, Zrinka - Zagreb : Hrvatsko društvo za biokemiju i molekularnu biologiju (HDBMB), 2006, 129-129

ISBN
953-95551-0-8

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation HDBMB2006

Mjesto i datum
Vodice, Hrvatska, 03.10.2006. - 07.10.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Hsp; MAPK; FORKO miševi
(Hsp; MAPK; FORKO mice)

Sažetak
Decline in androgens in males (andropause) and oestrogens in females (menopause) during aging increase the risk of osteoporosis, obesity, cardiovascular diseases, type 2 diabetes and of functional impairments of the central nervous system. We assessed the status of heat shock proteins (Hsps) and mitogen-activated protein kinases (MAPKs) in the hippocampus and the cortex of follitropin receptor knockout (FORKO) mice with enhanced LH and FSH together with estradiol and testosterone imbalance, as a consequence of FSH-R deletion. In the hippocampus, the expression of Hsp70 and Hsp25 was reduced in 20 months old FORKO males and both Hsps were significantly more down-regulated in age-matched females. In addition, female FORKO mice exhibited enhanced P-ERK already at 3 months of age while these changes become significant only in 20 months old male knockouts. In the cortex, the expression of Hsp70 and Hsp25 was significantly lower in older females. The signal intensity of P-ERK was strong in both genders. Functional characteristics of MAPKs are dependent on their subcellular localization. In the hippocampus of 12 months old FORKO females translocation of P-ERK to the nucleus decreased and more cytosolic P-ERK was found then in the age-matched wild type animals. On the other hand, nuclear P-JNK was significantly reduced whereas it was markedly enhanced in the mitochondria of the cortex of 20 months old FORKO males. In summary, enhanced gonadotropins alone or in combination with sex hormone imbalance in aging lead to biochemical changes in CNS in terms of disturbed Hsps expression, activation and subcellular distribution of MAPKs, and those changes could present a risk factor for neurodegeneration in menopause and late andropause.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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