Pregled bibliografske jedinice broj: 587652
Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant
Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant // Applied and environmental microbiology, 78 (2012), 19; 7120-7123 doi:10.1128/AEM.01794-12 (međunarodna recenzija, članak, znanstveni)
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Naslov
Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant
Autori
Schreier, Harold ; Dejtisakdi, Wipawee ; Escalante, Jose ; Brailo, Marina
Izvornik
Applied and environmental microbiology (0099-2240) 78
(2012), 19;
7120-7123
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
metabolism; Planctomycetes; mutagenesis; gluconeogenesis
Sažetak
A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free media demonstrating a gluconeogenic role for pckA in P. limnophilus.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
