Naslov
Cytokeratin-20 as a marker for detection of circulating tumor cells in preoperative and postoperative blood samples from colorectal cancer patients

Autori
Šamija, Ivan ; Lukač, Josip ; Končar-Mubrin, Mirjana ; Kirac, Iva ; Kovačević, Dujo ; Kusić, Zvonko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
22nd Biennial Congress of the European Association for Cancer Research, Proceedings Book / Eggermont, Alexander M.M. - : Elsevier, 2012, S178-S178

Skup
22nd Biennial Congress of the European Association for Cancer Research

Mjesto i datum
Barcelona, Španjolska, 07.07.2012. - 10.07.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cytokeratin-20; colorectal cancer; circulating tumor cells

Sažetak
Background: In this study cytokeratin-20 (CK20) was evaluated as a marker for detection of circulating colorectal cancer cells by real-time RT-PCR and influence of surgical tumor resection on the presence of circulating colorectal cancer cells was analyzed. Material and methods: Blood samples were taken from 95 colorectal cancer patients before and after tumor resection. Blood samples from 23 healthy volunteers were analyzed as controls. RNA was isolated from mononuclear cell fraction of the blood and assayed by real-time RT-PCR for the expression of CK20. Results: One out of 23 healthy volunteers was positive for CK20. Out of 95 colorectal cancer patients, 25 were positive for CK20 before surgery and 23 were positive for CK20 after surgery. Nine patients that were positive before surgery became negative after surgery, while 15 patients that were negative before surgery became positive after surgery. A statistically significant trend of increase in the proportion of CK20 positive blood samples with increasing stage of the disease was observed for preoperative, but not for postoperative blood samples. Conclusions: This study confirmed that CK20 is a specific marker for detection of circulating colorectal cancer cells and association with clinical stage indicates that it might have prognostic value. We have also shown that surgical tumor resection can both reduce and induce the presence of circulating colorectal cancer cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

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