Naslov
The cholinergic and non-cholinergic effects of organophosphates and oximes in cultured human myoblasts

Autori
Katalinić, Maja ; Miš, Katarina ; Grubič, Zoran ; Kovarik, Zrinka ; Marš, Tomaž

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
11th International Meeting on Cholinesterases, Kazan, Rusija, Book of Abstracts / Lushchekina, S. - : Kazan State University, 2012

Skup
11th International Meeting on Cholinesterases

Mjesto i datum
Kazan, Ruska Federacija, 04.06.2012. - 09.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
human myoblasts ; muscle regeneration ; HSP27 ; IL-6

Sažetak
The proposed research was set on investigation of the cholinergic and non-cholinergic effects of OPs/oximes on human myoblasts as precursors of muscle regeneration. Namely, as cholinergic effects are linked to AChE activity, non-cholinergic actions might directly or indirectly modify functioning of the complex intracellular mechanisms and in this way importantly contribute to the effects of the OPs/oximes. With this regard we studied the effects of OPs/oximes on the cell response mechanisms like expression of heat shock proteins and secretion of IL-6 and on differentiation markers like expression of Na+/K+ ATPase. Preliminary results showed that the cholinergic effects of OPs/oximes on the inhibition/reactivation of AChE in human myoblasts were as expected. In all combinations tested these effects were restricted to the AChE catalytic activity and did not interfere with the AChE mRNA expression even if AChE hydrolytic activity was significantly affected. However, beside these effects we also observed significant changes in the protein levels of HSP 27 and in the secretion of IL-6 after OP treatments. In our experiments done up to now secretion of IL-6 was increased when the level of HSP 27 was decreased and vice versa 24 hours post OPs treatment. This link between the observed responses to OPs (IL-6 vs. HSP 27) suggests a signalling pathway which is affected by OPs and which regulates both HSP 27 and IL-6 expression with an opposite trend. Changes of HSP 72 and IL-6 level were attenuated when oximes were applied as pretreatment suggesting that phosphorylation by OPs and dephosphorylation by oximes takes place also in these, yet putative non-cholinergic mechanisms. On the other hand, it seems that all observed effect were common for OPs as well as for oximes tested which indicated that the effects themselves are more likely associated to this specific group of compounds rather than a specific compound itself.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA