Naslov
Mathematical modeling of maize starch liquefaction catalyzed by α-amylases from Bacillus licheniformis: effect of calcium, pH and temperature

Autori
Vrsalović Presečki, Ana ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Đurđa

Izvornik
Bioprocess and biosystems engineering (1615-7591) 36 (2013), 1; 117-126

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
α-amylase ; liquefaction ; modeling ; enzyme inactivation ; starch hydrolysis

Sažetak
The first step of starch hydrolysis, i.e. liquefaction has been studied in this work. Two commercial α-amylases from Bacilllus licheniformis, known as Termamyl and Liquozyme have been used for this purpose. Using starch as the substrate, kinetics of both enzymes has been determined at optimal pH and temperature (pH 7, T = 80 °C) and at the 65 °C and pH 5.5. Michaelis- Menten model with uncompetitive product inhibition was used to describe enzyme kinetics. Mathematical models were developed and validated in the repetitive batch and fed-batch reactor. Enzyme inactivation was described by the two step inactivation model. All experiments were performed with and without calcium ions. The activities of both tested amylases are approximately one hundred times higher at 80 °C than at 65 °C. Lower inactivation rates of enzymes were noticed in the experiments performed at 65 °C without the addition of calcium than in the experiments at 80 °C. Calcium ions in the reaction medium significantly enhance amylases stability at 80°C and pH 7. At other process conditions (65 °C and pH 5.5) a weaker calcium stabilizing effect was detected.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

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