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Pregled bibliografske jedinice broj: 585356

A novel application of CIM monolithic supports for concentrating Potato spindle tuber viroid from water samples

Ruščić, Jelena; Gutiérrez-Aguirre, Ion; Urbas, Lidija; Kramberger, Petra; Mehle, Nataša; Škorić, Dijana; Barut, Miloš; Ravnikar, Maja; Krajačić, Mladen
A novel application of CIM monolithic supports for concentrating Potato spindle tuber viroid from water samples // Book of abstracts MSS2012 - 5th Monolith Summer School and Symposium Applications in Biochromatography, Bioconversion and Solid Phase Synthesis / Štrancar, Aleš (ur.).
Portorož, Slovenija; Ajdovščina, Slovenija, 2012. str. 46-46 (poster, međunarodna recenzija, sažetak, znanstveni)

CROSBI ID: 585356 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
A novel application of CIM monolithic supports for concentrating Potato spindle tuber viroid from water samples

Autori
Ruščić, Jelena ; Gutiérrez-Aguirre, Ion ; Urbas, Lidija ; Kramberger, Petra ; Mehle, Nataša ; Škorić, Dijana ; Barut, Miloš ; Ravnikar, Maja ; Krajačić, Mladen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts MSS2012 - 5th Monolith Summer School and Symposium Applications in Biochromatography, Bioconversion and Solid Phase Synthesis / Štrancar, Aleš - , 2012, 46-46

Skup
MSS2012 - 5th Monolith Summer School and Symposium

Mjesto i datum
Portorož, Slovenija; Ajdovščina, Slovenija, 01.06.2012. - 06.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
viroid; kromatografija; monolitni nosač; koncentriranje
(viroid; chromatography; monolithic support; concentrating)

Sažetak
Potato spindle tuber viroid (PSTVd) is the causal agent of a number of agriculturally important diseases. It is a single-stranded, circular and uncapsidated RNA molecule with 359 nucleotides and no coding capacity. Because of its complex secondary/tertiary structure it is very stable ex vivo and it is easily transmitted mechanically by contaminated hands, tools, machinery, etc. In this work, we describe the development and optimization of a method for concentrating PSTVd using CIM monolithic supports. The ion-exchange chromatography on CIMacTM DEAE columns resulted in up to 30% PSTVd recovery whilst the hydrophobic interaction chromatography on CIMacTM C4 columns improved it up to 60%. Based on these results, a CIM® C4 HLD-1 (High Ligand Density) tube monolithic column was selected for further experiments. One-liter-water samples were mixed with different viroid quantities and loaded onto the column. By using RT-qPCR, the viroid RNA was quantified in the elution fraction (≈ 5 mL) indicating that 70% of the viroid was recovered and concentrated by at least two orders of magnitude. This approach would be helpful in screening irrigation waters and/or hydroponic systems’ nutrient solutions for the presence of PSTVd.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Projekti:
119-1191192-1222 - Molekularna varijabilnost biljnih patogena (Krajačić, Mladen, MZOS ) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Dijana Škorić (autor)

Avatar Url Jelena Ruščić (autor)

Avatar Url Mladen Krajačić (autor)

Citiraj ovu publikaciju:

Ruščić, Jelena; Gutiérrez-Aguirre, Ion; Urbas, Lidija; Kramberger, Petra; Mehle, Nataša; Škorić, Dijana; Barut, Miloš; Ravnikar, Maja; Krajačić, Mladen
A novel application of CIM monolithic supports for concentrating Potato spindle tuber viroid from water samples // Book of abstracts MSS2012 - 5th Monolith Summer School and Symposium Applications in Biochromatography, Bioconversion and Solid Phase Synthesis / Štrancar, Aleš (ur.).
Portorož, Slovenija; Ajdovščina, Slovenija, 2012. str. 46-46 (poster, međunarodna recenzija, sažetak, znanstveni)
Ruščić, J., Gutiérrez-Aguirre, I., Urbas, L., Kramberger, P., Mehle, N., Škorić, D., Barut, M., Ravnikar, M. & Krajačić, M. (2012) A novel application of CIM monolithic supports for concentrating Potato spindle tuber viroid from water samples. U: Štrancar, A. (ur.)Book of abstracts MSS2012 - 5th Monolith Summer School and Symposium Applications in Biochromatography, Bioconversion and Solid Phase Synthesis.
@article{article, author = {Ru\v{s}\v{c}i\'{c}, Jelena and Guti\'{e}rrez-Aguirre, Ion and Urbas, Lidija and Kramberger, Petra and Mehle, Nata\v{s}a and \v{S}kori\'{c}, Dijana and Barut, Milo\v{s} and Ravnikar, Maja and Kraja\v{c}i\'{c}, Mladen}, editor = {\v{S}trancar, A.}, year = {2012}, pages = {46-46}, keywords = {viroid, kromatografija, monolitni nosa\v{c}, koncentriranje}, title = {A novel application of CIM monolithic supports for concentrating Potato spindle tuber viroid from water samples}, keyword = {viroid, kromatografija, monolitni nosa\v{c}, koncentriranje}, publisherplace = {Portoro\v{z}, Slovenija; Ajdov\v{s}\v{c}ina, Slovenija} }
@article{article, author = {Ru\v{s}\v{c}i\'{c}, Jelena and Guti\'{e}rrez-Aguirre, Ion and Urbas, Lidija and Kramberger, Petra and Mehle, Nata\v{s}a and \v{S}kori\'{c}, Dijana and Barut, Milo\v{s} and Ravnikar, Maja and Kraja\v{c}i\'{c}, Mladen}, editor = {\v{S}trancar, A.}, year = {2012}, pages = {46-46}, keywords = {viroid, chromatography, monolithic support, concentrating}, title = {A novel application of CIM monolithic supports for concentrating Potato spindle tuber viroid from water samples}, keyword = {viroid, chromatography, monolithic support, concentrating}, publisherplace = {Portoro\v{z}, Slovenija; Ajdov\v{s}\v{c}ina, Slovenija} }

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