Naslov
Protein expression profile of Mytilus galloprovincialis haemolymph and its modifications following exposure to heavy metals

Autori
Lorena Perić, Lara Boatti, Francesco Marsano, Aldo Viarengo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 154 (1) / - : Elsevier, 2009, S18-S19

Skup
New European Society for Comparative Physiology and Biochemistry 26th ESCPBnew

Mjesto i datum
Innsbruck, Austrija, 09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Mytilus galloprovincialis; haemolymph protein; metal exposure; proteomics

Sažetak
Haemolymph represents the circulatory fluid of mollusks with an open circulatory system. The majority of studies of the mussels Mytilus galloprovincialis haemolymph have mainly been focused on the function of blood cellular component and humoral factors related to immune defense, while data on the protein composition has recently started to emerge. Thus, the goal of the present study was to investigate the mussel’s haemolymph proteome, utilizing proteomics techniques such as two-dimensional electrophoresis (2DE) in combination with mass spectrometry. 2DE under denaturing conditions revealed the presence of several tightly closed and highly abundant protein bands of (30-45 kDa) and with pI values from (4.0 – 6.0). With respect to the above characteristics, the dominant protein component matched hystidine rich glycoprotein (HRG) previously isolated from haemolymph of M. edulis on the basis of its highly expressed metal binding properties. The protein spot was identified by ESI-QTOF MS/MS and the database search revealed its high homology with the extrapallial protein (EP) which was isolated from extrapallial fluid of mussel M. edulis and could have a role in Ca2+ - transport and as a shell matrix protein. We also investigated the protein expression in M. galloprovincialis haemolymph, following in vivo exposure to sublethal concentrations of heavy metals known to induce the formation of reactive oxygen species (ROS), which can cause carbonylation of proteins. Quantization of carbonylated proteins in M. galloprovincialis haemolymph could provide further insight in the effects of oxidative stress on the proteome of this bivalve species.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

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