Naslov
Identification and quantification of plant hormones auxins in Brassica rapa seedlings using GC-MS

Autori
Šamec, Dunja ; Smolko, Ana ; Ludwig-Müller Jutta ; Salopek-Sondi, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th International Chromatography School- Book of Abstracts / Ukić, Š ; Bolača T. - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2012, 47-47

ISBN
978-953-6470-58-7

Skup
13th International Chromatography School

Mjesto i datum
Zagreb, Hrvatska, 18.06.2012. - 19.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
auxins ; plant hormones ; GC-MS

Sažetak
Plant hormones (phytohormones) play an important role in regulating the growth, metabolism and development of plants. Among them auxin indole-3-acetic acid (IAA) has been firstly identified and studied since Darwin’s time. In plants, auxins occur in a very low amount. Therefore, research in the field of biochemical processes in which auxins are involved includes application of highly sensitive analytical methods for their identification and quantification. In research presented here we used GC-MS (gas chromatography–mass spectrometry) method for identification and quantification of IAA, indol-3-propionic (IPA) and indole-3- butyric (IBA) acids in Brassica rapa seedlings upon treatments with 100 mM of jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA) in comparison to controls. For auxin identification samples were prepared by liquid/liquid extraction followed by solid- phase extraction (SPE), after which samples were methylated using diazomethane. In a full scan mode that was used for auxin identification we found IAA in all samples, while occurrence of IBA and IPA were only sporadically determined and could not be reproducible. Quantification of IAA was done in μSIS mode because of its higher sensitivity, by adding [13C6] IAA as internal standard. In addition to free IAA, total IAA was determined after alkaline hydrolysis with 7N NaOH at 100 °C for 3 h. We found significantly higher amount of free and conjugated IAA in samples treated with jasmonic acid (free: 11.10±1.55 ng/mg dw ; conjugated: 126.62±3.90.12 ng/mg dw) in comparison to control (free: 4.63±0.21 ng/mg dw ; conjugated: 43.47±5.54 ng/mg dw) samples. Treatments with SA and ABA did not significantly influence IAA content in comparison to controls. Sporadically identification of long-chained auxins IPA and IBA confirmed their potential occurrence in B. rapa plants but detailed protocol for identification needs to be established.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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