Naslov
DISTRIBUTION OF TISSUE METALS IN THE BRAIN OF RAT STRAINS WITH GENETICALLY DIFFERENT SUSCEPTIBILITY TO EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS

Autori
Tota, Marin ; Broznić, Dalibor ; Grubić-Kezele, Tanja ; Jakovac, Hrvoje ; Barac-Latas, Vesna ; Milin, Čedomila ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEBS 3+ Meeting, Book of Abstracts / - , 2012

Skup
"From molecules to life and back" FEBS 3+ Meeting

Mjesto i datum
Opatija, Hrvatska, 13.06.2012. - 16.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
autoimmune encephalomyelitis; metallothioneins; zinc; iron; copper

Sažetak
Albino Oxford (AO) rats, compared to the Dark Agouti (DA) strain, exhibit lower susceptibility to the induction of experimental autoimmune encephalomyelitis (EAE). Mechanisms include the differences in peripheral response to immunization and those linked with the CNS milieu, which contribute to limit the injury. In the search for factors related to these differences, previously we found that these strains significantly differ in the constitutive gene and protein expression of the cysteine rich proteins-metallothioneins I and II (MTs) that maintain the metal ion homeostasis and have marked anti-inflammatory and neuroprotective properties. In attempt to enlarge these findings, in this study we correlated the protein expression of MTs with tissue concentrations of Zn+2, Cu+2 and Fe+2 in the brain and its regions, which were highly sensitive to autoimmune attack - i.e. in hippocampus, cerebellum and lumbar spinal cord. Rats were immunized with bovine brain homogenate (BBH) emulsified in Freund's Complete Adjuvant (CFA) or with CFA only. On days 7 and 12 after immunization the tissue concentrations of Zn2+, Cu2+ and Fe2+ were estimated in the whole brain and its regions, and in the spinal cord by inductively coupled plasma spectrometry. AO rats did not show any clinical signs of EAE after immunization with BBH+CFA, but MTs protein expression were upregulated in some regions of CNS. This strain of rats had, however, greater basal levels of Zn+2 and Cu+2 in hippocampus and lower concentrations of Zn+2, Cu+2 and Fe+2 in spinal cord. Besides, in comparison to EAE-susceptible DA rats they were able to better maintain the metal homeostasis in the brain and spinal cord tissue after immunization. The data point to genetic differences in the mechanisms that during the autoimmune attack through activation of MTs and metal uptake or release ensure protection of the most sensitive brain regions against oxidative and nitrosative injuries.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

POVEZANOST RADA