Naslov
Effect of diets enriched with pumpkin-seed, olive and fish oil or lard on mice liver nonpolar lipids and expression of lipin and PPARα after partial hepatectomy

Autori
Čanadi Jurešić, Gordana ; Broznić, Dalibor ; Marinić, Jelena ; Tota, Marin ; Čedomila, Milin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
From molecules to life and back ; FEBS3+ Meeting ; Book of Abstracts / Dumić, Jerka ; Kovarik, Zrinka ; Varljen, Jadranka - Rijeka : Hrvatsko Društvo za Biotehnologiju, 2012, 183-183

ISBN
978-953-95551-4-4

Skup
From molecules to life and back ; FEBS3+ Meeting ;

Mjesto i datum
Opatija, Hrvatska, 13.06.2012. - 16.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
partial hepatectomy; oils; lipids; lipin; PPARa

Sažetak
The best experimental model for the study of liver regeneration is partial hepatectomy (pHx), in which part of the liver is surgically removed. Fast liver growth, following pHx is a well defined process, that involves the concerted action of extra and intracellular factors resulting in cell replication and its inhibition at the time when the entire liver mass is restored. During regeneration, liver transiently accumulates large amounts of triglyceride fat. One of the factors involved in hepatocellular fat accumulation following pHx could be lipin 1. This protein is defined as the bridge between hepatic glycerophospholipid biosynthesis and lipoprotein metabolism. It activates expression of many genes involved in mitochondrial fatty acid oxidative metabolism, among other, via transcriptional activation of the gene encoding PPARα. In this study, the changes in the expression level of mice hepatic lipin 1 and PPARα were evaluated and compared with the changes in the content of nonpolar liver lipids. As nutritional factors influence the process of liver regeneration, effect of diets enriched with different oils or lard and their impact to gene expression was also evaluated. For that purpose, male C57/Bl6 mice, aged 8-10 weeks, were fed by diets enriched either with pumpkin-seed, olive or fish oil or lard (5% addition to standard pellet, w/w) during the 3 weeks and afterwards subjected to 1/3 pHx. 2, 4, 24, 48 and 168 hours later mice were sacrificed and the remaining liver analysed. Total liver lipids were extracted and then separated into fractions by solid-phase extraction. Real-time reverse transcription-PCR was performed to quantitate total lipin and PPARα expression levels. Each of the experimental diets affected liver regeneration differently. In all diets, except in fish oil enriched diets, regenerating liver accumulated extra fat (increment of neutral lipid fraction) in first few days after pHx, with the highest value at 24 hours after pHx. Fish oil enriched diet provoked the fastest liver regeneration (after 48 hours >90% of the original mass) and simultaneously, reflected itself in the lowest total liver lipid content. Lipin expression levels correlates with PPARα expression levels, although each of the diet provokes different response. Besides, we demonstrate that the expression of lipin 1 can be induced by pHx

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija

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