Naslov
Micropropagation and genotypic diversity of wild cherry (Prunus avium L.) in the area of its natural distribution in Croatia

Autori
Tančeva Crmarić, Olivera ; Štambuk, Snježana ; Šatović, Zlatko ; Kajba, Davorin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Croatian genetic society, 3rd congress of croatian geneticists / Franekić, Jasna ; Garaj-Vrhovac, Verica - Zagreb : Hrvatsko genetičko društvo, 2012, 85-85

ISBN
978-953-57128-0-0

Skup
Croatian genetic society, 3rd congress of croatian geneticists

Mjesto i datum
Krk, Hrvatska, 13.05.2012. - 16.05.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Wild cherry ; micropropagation ; genotypic diversity

Sažetak
From established clonal seed orchards of wild cherry (Prunus avium L.) in the process of in vitro production 24 genotypes (selected plus trees) were introduced. The possibility of optimization of routine micropropagation methods of clones at all stages was explored. Some aseptic techniques were established that allow the introduction of the initial culture throughout the year. Specific structure of nutrient media and unique combination of growth regulators are determined in in vitro production stages, which allow to obtain high-quality plants with very good survival during the acclimatization process. For micropropagation were used BAP–1, 0 mg/L, Kinetin- 0, 5 mg/L and IAA-0, 5 mg/L, which resulted in multiplication rate of 3-9 with a plant height from 1, 5 to 3, 0 cm. Microplants rooting was achieved by a combination of IAA-2, 0 mg/L, IBA-2, 0 mg/L with the addition of GA3-0, 2 mg/L. The clones were analyzed by 15 selected microsatellite markers (SSR), chosen by the ECPGR. A wealth of allelic variations was found in SSR loci, while a high degree of polymorphism confirmed the existence not only of extensive morphological but also a very significant genetic diversity. Based on pairwise proportion of shared allele distance (DPSAM) the average genetic distance of 0, 573 was calculated. The smallest genetic distance (DPSAM = 0, 100) was recorded between the genotypes 'KP2' and 'KP5' (region Koprivnica), which coincided in 27 out of 30 alleles, whereas the largest genetic distance (DPSAM = 0, 933) was found between the genotypes 'Đu2' (Bjelovar region) and 'L3' (Zagreb region), which differed in 28 out of 30 alleles. The genetic distance matrix, based on pairwise proportion of shared allele distance (DPSAM), did not show a clear classification of wild cherry individuals with regard to their origin, i.e. region. The analysis of molecular variance (AMOVA) revealed a significantly higher percentage (95, 88 %) of the total microsatellite diversity caused by the differences among the invidividuals within the regions, compared to that caused by the differences between the studied regions (4, 12 %). The  - statistics, amounting to 0, 041, was highly significant (P < 0.01) and indicates the existence of specific regional structurality of genetic diversity. It is presented by the axes of factorial correspondence analysis (FCA). The first axis explains 63, 76 % of the total inertia and discriminates the individuals from the Zagreb region from those from the Bjelovar and Koprivnica regions, while the second axis with 36, 24 % discriminates the individuals from the Bjelovar region from those in Koprivnica region. The existence of specific regional structuring is shown by factorial correspondence analysis (FCA) and the genetic diversity of wild cherry was determined in the disseminated area in northwestern Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Šumarstvo, Biotehnologija

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