Naslov
Summary of the first round analyses of the third International Swine CD Workshop

Autori
Haverson, Karin ; Saalmüller, Armin ; Chen, Z. ; Huang, C.A. ; Simon, A. ; Boersma, W.J.A. ; Zwart, R. ; Niewold, T.A. ; Thacker, E. ; Llanes, D. ; Perez de la Lastra, J.M.P. ; Engelhardt, H. ; Ezquerra, A. ; Alonso, F. ; Dominguez, J. ; Ledbetter, J.A. ; Grosmaire, L. ; Lee, R. ; Nielsen, J. ; Salmon, H. ; Valpotić, Ivica ; Lacković, Gordana ; Šver, Lidija ; Summerfield, A. ; Khanna, K.V.

Izvornik
Veterinary Immunology and Immunopathology (0165-2427) 80 (2001), 1-2; 25-34

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, bibliografija, znanstveni

Ključne riječi
Monoclonal antibodies; cluster of differentiation; flow cytometry; swine

Sažetak
The reactivity of 152 monoclonal antibodies submitted to the Third International Swine CD workshop, together with 41 internal standards, was analysed by the flow cytometry on 29 different pig cell targets as well as 2 human cell targets as a means of establing suitable panels of monoclonal antibodies for more detailed clustering analyses by the various subsections of the workshop. Results were collected either without futher gating, with gating based on FS/SS characteristics or with gating based on the co-expression of a reference antibody in 2-colour flow cytometry. The CD or SwC reactivity of the internal standards had been established in previous workshops. Data sets were subsequently analysed by statistical clustering using the Leukocyte Typing Database IV software. The resulting 18 cluster groups were allocated to the appropriate second round sections of the workshop, after reviewing the overall cellular reactivity of each cluster as well as the specifity of known standards which clustered in a group.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA