Naslov
Coenzyme regeneration in hexanol oxidation catalyzed by alcohol dehydrogenase

Autori
Vrsalović Presečki, Ana ; Makovšek, Katja ; Vasić- Rački, Đurđa

Izvornik
Applied biochemistry and biotechnology (0273-2289) 167 (2012), 3; 595-611

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
yeast ; coenzyme regeneration ; enzyme kinetics ; alcohol dehydrogenase ; modeling ; alcohol oxidation

Sažetak
The enzymatic ways of coenzyme regeneration include the addition of a second enzyme to the system or the addition of the co-substrate. In the present study both methods of enzymatic coenzyme (NAD+) regeneration were studied and compared in the reaction of hexanol oxidation catalyzed by alcohol dehydrogenase (ADH). As a source of ADH, commercial isolated enzyme and the whole baker's yeast cells were used. First, coenzyme regeneration was employed in the reaction of acetaldehyde reduction catalyzed by the same enzyme that catalyzed the main reaction, and then NAD+ regeneration was applied in the reaction of pyruvate reduction catalyzed by L-lactate dehydrogenase. Hexanal was obtained as the product of hexanol oxidation catalyzed by isolated ADH while hexaonic acid was detected as a product of the same reaction catalyzed by baker's yeast cells. All of the used biocatalysts were kinetically characterized. The mass reactions were described by the mathematical models. All models were validated in the batch reactor. 100 % hexanol conversion was obtained using permeabilized yeast cells using both methods of cofactor regeneration. By using isolated enzyme ADH, the higher conversion was achieved in a system with cofactor regeneration catalyzed by L-LDH.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA