Naslov
Modification of protein-gliadine-by phosphine

Autori
Steiner-Biočić, Ivka ; Bešlo, Drago

Izvornik
Acta pharmaceutica (1330-0075) 44 (1994), 3; 265-268

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
gliadine modification ; phosphine

Sažetak
Modification of protein- gliadine - by phosphine (PH3) has been investigated. As a substrate for investigation, gliadine was isolated from wheat. Phosphine is a pesticide used for wheat protection against insects. Gliadine contains 4 different subunits: α, β, γ, ω. Protein concentration was determined by the Lowry method. Subunits of gliadine were determined by the Weber and Osborn method using SDS-gel electrophoresis. The investigations detected changes in β-subunits of the PH3- treated gliadine as compared to the not treated protein. β-subunits appeared on the electrophoregram in two fractions. It is well known that gliadine β-subunits contain relatively a high percentage of disulphide cross-links. Phosphine is a strong reductive reagent that causes reduction of disulphide bonds. Two fractions of β-subunits can be explained as being structure changes on disulphide bonds by PH3.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA