Naslov
Potentiometric Study of α-Amylase Kinetics Using a Platinum Redox Sensor

Autori
Sakač, Nikola ; Sak-Bosnar, Milan

Izvornik
International Journal of Electrochemical Science (1452-3981) 7 (2012), 4; 3008-3017

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
α-amylase; kinetics; starch-triiodide; direct potentiometry; redox sensor

Sažetak
A potentiometric study of α-amylase kinetics using a platinum redox sensor is described in this paper. The sensor measured the amount of triiodide ion released from a starch-triiodide complex after biocatalytic starch degradation, which was correlated with the α-amylase activity. The response characteristics of the platinum redox sensor to the triiodide ion and the working parameters for the starch hydrolysis have been optimized. The kinetics of starch degradation using α-amylase have been measured by direct potentiometry. The two theoretical models for α-amylase kinetics, Michaelis-Menten and Lineweaver-Burk, were calculated from the experimental data and optimized using Solver. The calculated parameter values for α-amylase were Vmax = 2.99E-5 and Km = 0.4862 for the Michaelis-Menten model, and Vmax = 3.31E-05 and Km = 0.5771, Slope = 17449, for the Lineweaver-Burk model.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA