Naslov
Macrophages secreted cytokines regulate perforin expression in the first trimester pregnancy decidua

Autori
Sotošek, Vlatka ; Laškarin, Gordana ; Štrbo, Nataša ; Bogović, Tatjana ; Randić, Ljiljana ; Vlastelić, Ivica ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1. kongres Hrvatskog društva fiziologa s međunarodnim sudjelovanjem : knjiga sažetaka = Abstract book / Vitale, B. - Osijek : Hrvatsko društvo fiziologa, 2000, P-17

Skup
Kongres Hrvatskog društva fiziologa s međunarodnim sudjelovanjem (1 ; 2000)

Mjesto i datum
Osijek, Hrvatska, 14.09.2000. - 16.09.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
interleukin-10; interleukin-12; interleukin-15; perforin; decidual macrophages

Sažetak
Recently it has been suggested that optional cytokines interaction at materno-fetal interface are responsible for successful pregnancy. Cytokines IL-4, IL-6, IL-10, IL-13 have been suggested to be beneficial while IFN , IL-2, IL-12, TNF are considered detrimental to the maintenance of pregnancy. Decidual large granular lymphocytes (dLGL), which contain high level of cytolytic protein perforin (P), serve an immune surveillance function protecting from microbial attack, malignant transformation and cytotrophoblast invasion. Our previous results showed that the interactions between dLGL and decidual macrophages (dM) play an important role in regulation of perforin: perforin can be upregulated by the addition either dM or their supernatants. The aim of our study was to investigate whether this regulation of P expression by dM is mediated by cytokines IL-10, IL-12 and IL-15. Antibodies anti IL-10, but not anti IL-12, were efficient in preventing the effects of dM on maintaining the P level in DL. Surprisingly, effects of dM and their supernatants on P expression in DL can be blocked completely by the addition of monoclonal anti IL-15 antibody. Contrary, P expression in PBL can not be blocked by the addition of anti IL-15 antibody. The results are pointing to the importance of macrophages and IL-15 in the control of activation of perforin mediated cytolytic pathway in decidua.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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