Naslov
Isoamyl acetate synthesis in imidazolium-based ionic liquids using packed bed enzyme microreactor

Autori
Cvjetko, Marina ; Vorkapić-Furač, Jasna ; Žnidaršič-Plazl, Polona

Izvornik
Process biochemistry (1991) (1359-5113) 47 (2012), 9; 1344-1350

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ionic liquids; packed bed microbioreactor; Candida antarctica lipase B; isoamyl acetate; esterification

Sažetak
The acylation of isoamyl alcohol with acetic anhydride catalyzed by immobilized Candida antarctica 15 lipase B was studied in ionic liquids (ILs) based on quaternary imidazolium cations with alkyl, alkenyl, alkynyl, benzyl, alkoxyl or N-aminopropyl side chains. Among tested ILs, the highest enzyme activity together with the highest isoamyl acetate yield were obtained in [hpdmim][Tf2N]. No loss of lipase B activity was observed during one-month incubation in this hydrophobic IL without the presence of substrates. Isoamyl acetate synthesis using [hpdmim][Tf2N] as solvent was further studied in a continuously operated miniaturized enzymatic packed bed reactor at various flow rates and temperatures. Up to 92% soamyl acetate yield could be obtained within 15 min by using 0.5 M acetic anhydride and 1.5 M isoamyl alcohol inlet concentrations at 55°C, corresponding to the volumetric productivity of 61 mmol l-1 min-1 , which is to the best of our knowledge the highest reported so far for this reaction. No decrease in productivity was experienced during the subsequent runs of continuous microbioreactor operation performed within 14 consecutive days. The benefits of reactor miniaturization along with the green solvent application were therefore successfully exploited for the development of a sustainable flavour ester production.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA