Naslov
Cadmium and Zinc Induced Similar Changes in Protein and Glycoprotein Patterns in Tobacco (Nicotiana tabacum L.) Seedlings and Plants

Autori
Peharec Štefanić, Petra ; Šikić, Sandra ; Cvjetko, Petra ; Balen, Biljana

Izvornik
ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU (0004-1254) 63 (2012); 321-335

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
glycoproteins; heavy metals; lectins; tobacco; protein pattern

Sažetak
The effects of 10 and 15 µmol L-1 cadmium (Cd), a nonessential toxic element and 25 and 50 µmol L-1 zinc (Zn), an essential micronutrient, on proteins and glycoproteins of Nicotiana tabacum L. seedlings and plants were investigated after exposure to each metal alone or to their combinations. Changes in only few polypeptides related to heavy metal treatments were observed in tobacco seedlings and leaves of adult plants, while the greatest change in total soluble protein pattern was observed in plant roots. Differences between control and treated tobacco tissues were more pronounced in the glycoprotein pattern, which was analysed by application of different lectins. The majority of the detected glycoproteins in leaves and roots of adult plants can be considered as a result of enhanced glycosylation due to heavy metal stress. The difference in glycoproteins between Cd and Zn application on tobacco seedlings and adult plants could not be determined since enhanced glycosylation was noticed after treatment with either metal alone or in combination. Therefore, it can be concluded that both metals induced N- and O-glycosylation as a result of changed environmental conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA