Naslov
Expression of cytolytic protein - perforin in peripheral blood, synovial fluid and synovial tissue lymphocytes in patients with rheumatoid arhtiritis

Autori
Gulan, Gordan ; Ravlić-Gulan, Jagoda ; Sotošek, Vlatka ; Štrbo, Nataša ; Matovinović, Damir ; Nemec, Boris ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the Fourth International Meeting "Mechanisms in Local Immunity" ; u: Periodicum Biologorum. Supplement 99 (1997) / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1998, 80-80

Skup
International Meeting "Mechanisms in Local Immunity" (4 ; 1998)

Mjesto i datum
Opatija, Hrvatska, 16.09.1998. - 19.09.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
rheumatoid arthritis; perforin; synovial fluid; synovial tissue

Sažetak
Rheumatoid arthritis (RA) is a progressive, destructive disease characterized by synovial hyperplasia, inflammation, and abnormal immune response. The causative agents that initiate the disease and the underlying mechanisms that result in damage, remain largely obscure. To test the hypothesis that cytotoxicity plays an important role in RA, in this study we used the marker that correlate with the function of cytolytic lymphocytes-cytolytic protein well known as perforin, which is located in the granules of cytotoxic lymphocytes. This protein appear to be involved in the killing processes as it is expressed only in activated killer cells and its expression is tightly regulated. Using direct and indirect immunoflorescence we measured the percentage of P+ cells in peripheral blood, synovial fluid (SF) and synovial membrane (SM) of patients with acute phase of RA. The percentages of P+ cells were compared with those obtained from RA patients in remission phase and patients with osteoarthrosis as well as with patients under immunosuppressive therapy. In peripheral blood, the percentage of P+ cells was significantly higher in patients with acute RA (47, 1%) in comparison with healthy individuals (26.2% ; p 0, 001), chronic RA (28.9 ; p 0, 01), osteoarthrosis (24, 6% ; p 0, 001). All investigated groups had higher percentage of P+ cells comparing with patients treated with immunosuppressive therapy. In the joints, the percentage of P+ cells was significantly higher in acute RA in comparison with chronic RA and OA (p 0, 001) as has been shown at systemic level as well, but the chronic RA patients had significantly higher percentage of P+ cells in SF and SM in comparison with OA. The obtained results provide the evidence implicating P+ cells in the pathogenesis of RA and suggest that the P+ cells are included in possible mechanisms of joint and tissue destruction with probably different assignment in systemic and local level.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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