Naslov
A Molecular Peptide Beacon for the Ratiometric Sensing of Nucleic Acids

Autori
Wu, Junchen ; Zou, Ying ; Sicking, Wilhelm ; Piantanida, Ivo ; Yi, Tao ; Schmuck, Carsten

Izvornik
Journal of the American Chemical Society (0002-7863) 134 (2012), 4; 1958-1961

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
peptide beacon; DNA sensing; bioorganic chemistry; cell imaging

Sažetak
In conclusion, we have demonstrated that the pyrenefunctionalized oligopeptide 1 can be used as a molecular peptide beacon to sense ds-DNA. Upon binding to nucleic acids 1 undergoes a conformational change from a folded to an extended form which switches the fluorescence of 1 from an excimer emission to a monomer emission. PB 1 can thus be used for the ratiometric sensing of different types of polynucleotides as binding to p(dA•dT)2 is preferred to G-C containing polynucleotides. Most importantly PB 1 is taken up by cells and can thus be used for the staining of nuclear DNA using fluorescence microscopy. As 1 does not possess any general cytotoxicity it might be of interest for applications in diagnostics.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA