Naslov
Enantioselective enzymatic oxidation with indirect electrochemical NAD+ regeneration

Autori
Schroeder, Iris ; Vasić-Rački, Đurđa ; Wandrey, Christian ; Liese, Andreas

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Reactive intermediates in organic and biological electrochemistry in honor of late professor Eberhard Steckhan / Yoshida, J. ; Peters, D.G. ; Workentin, M.S. - Washington (MD) : The Electrochemical Society (ECS), 2001, 00-00

Skup
The 199th Meeting of The Electrochemical Society

Mjesto i datum
Sjedinjene Američke Države, 25.03.2001. - 29.03.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
enantioselective enzymatic oxidation; electrochemical NAD regeneration

Sažetak
For production of 2-hydroxyketones and racemic resolution of vicinal diols glycerol dehydrogenase (GDH) [EC 1.1.1.6] from Cellulomonas species was used as production enzyme.1 Since GDH is NAD + dependent and strongly inhibited by its reduced form NADH, an effective and cost efficient cofactor regeneration is necessary. For this regeneration ABTS+.was used as oxidizing agent, which itself was prepared in situ by anodic oxidation. Though electrochemical cofactor regeneration needs no cosubstrate nor additional enzymes, until now it is not used in industrial biotransformations.2, 3 The kinetic parameters were determined and a kinetic model for the electrochemically driven enzymatic reaction was developed. Based on this model the ratios of the reactants were optimized and the model proved by experiments. Synthesis of 2-hydroxyketones and racemic resolution were performed in different reactor types, avoiding product inhibition by continuous extraction of the formed 2-hydroxyketones. GDH was retained by using ultrafiltration membranes. Lit: 1 A. Liese, M. Karutz, J. Kamphuis, C. Wandrey, U. Kragl, Biotech. Bioeng. 1996, 51, 544-550 2 A. Liese, K. Seelbach, C. Wandrey, Industrial Biotransformations, VCH Weinheim 2000 3 E. Steckhan, Top. Curr. Chem 1994, 170, 83-111 Financial support by BASF AG Ludwigshafen (Germany) is gratefully acknowledged.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA