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Pregled bibliografske jedinice broj: 564691

Validation of a microscale extraction and highthroughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia

Cuthbertson, Daniel; Piljac-Žegarac, Jasenka; Lange, Bernd Markus
Validation of a microscale extraction and highthroughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia // Biomedical chromatography, 26 (2012), 10; 1191-1195 doi:10.1002/bmc.2677 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 564691 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Validation of a microscale extraction and highthroughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia
(Validation of a microscale extraction and highthroughputUHPLC-QTOF-MS analysis method for huperzine A in Huperzia)

Autori
Cuthbertson, Daniel ; Piljac-Žegarac, Jasenka ; Lange, Bernd Markus

Izvornik
Biomedical chromatography (0269-3879) 26 (2012), 10; 1191-1195

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
alkaloid; Huperzia; huperzine A; mass spectrometry; microscale extraction

Sažetak
Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase inhibiting alkaloid, from as little as 3mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95% recovery. We also validated a novel UHPLC-QTOF-MS method for the high-throughput analysis of H. squarrosa extracts in only 6 min, which, in combination with the very low limit of detection (20 pg on column) and the wide linear range for quantification (20–10, 000pg on column), allow for a highly efficient screening of extracts containing varying amounts of HupA. Utilization of this methodology has the potential to conserve valuable plant resources.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija



POVEZANOST RADA

Projekti:
098-0982913-2829 - Molekularna regulacija biljnog razvitka (Salopek-Sondi, Branka, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Jasenka Piljac Žegarac (autor)

Poveznice na cjeloviti tekst rada:

doi onlinelibrary.wiley.com

Citiraj ovu publikaciju:

Cuthbertson, Daniel; Piljac-Žegarac, Jasenka; Lange, Bernd Markus
Validation of a microscale extraction and highthroughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia // Biomedical chromatography, 26 (2012), 10; 1191-1195 doi:10.1002/bmc.2677 (međunarodna recenzija, članak, znanstveni)
Cuthbertson, D., Piljac-Žegarac, J. & Lange, B. (2012) Validation of a microscale extraction and highthroughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia. Biomedical chromatography, 26 (10), 1191-1195 doi:10.1002/bmc.2677.
@article{article, author = {Cuthbertson, Daniel and Piljac-\v{Z}egarac, Jasenka and Lange, Bernd Markus}, year = {2012}, pages = {1191-1195}, DOI = {10.1002/bmc.2677}, keywords = {alkaloid, Huperzia, huperzine A, mass spectrometry, microscale extraction}, journal = {Biomedical chromatography}, doi = {10.1002/bmc.2677}, volume = {26}, number = {10}, issn = {0269-3879}, title = {Validation of a microscale extraction and highthroughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia}, keyword = {alkaloid, Huperzia, huperzine A, mass spectrometry, microscale extraction} }
@article{article, author = {Cuthbertson, Daniel and Piljac-\v{Z}egarac, Jasenka and Lange, Bernd Markus}, year = {2012}, pages = {1191-1195}, DOI = {10.1002/bmc.2677}, keywords = {alkaloid, Huperzia, huperzine A, mass spectrometry, microscale extraction}, journal = {Biomedical chromatography}, doi = {10.1002/bmc.2677}, volume = {26}, number = {10}, issn = {0269-3879}, title = {Validation of a microscale extraction and highthroughputUHPLC-QTOF-MS analysis method for huperzine A in Huperzia}, keyword = {alkaloid, Huperzia, huperzine A, mass spectrometry, microscale extraction} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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