Naslov
Micronucleus assay in freshwater invertebrates

Autori
Pavlica, Mirjana ; Klobučar, Goran I.V. ; Maguire, Ivana ; Erben, Radovan ; Papeš, Dražena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Crotox 2000 - Final Programme and Abstract Book / Prpić-Majić, Danica - Zagreb : Hrvatsko toksikološko društvo, 2000

Skup
Crotox 2000 - 2nd Croatian Congress of Toxicology

Mjesto i datum
Pula, Hrvatska, 09.04.2000. - 12.04.2000

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
micronucleus assay; water invertebrates

Sažetak
The number of micronuclei (MNi) in haemocytes of freshwater mussel Dreissena polymorpha Pall., freshwater snails Planorbarius corneus L. and Lymnaea stagnalis L., and freshwater decapod crustaceans Astacus astacus L. and Austropotamobius torrentium Schr. was determined in order to develop a simple and fast method for genotoxicity evaluation of freshwater environment. The number of spontaneous MNi and MNi induced after exposure to pentachlorophenol was analysed in haemocytes of freshwater molluscs. Investigations on genotoxicity of river Sava, downstream from Zagreb, were initiated on haemocytes of D. polymorpha by micronucleus and comet-test. So far, only the spontaneous level of MNi in haemocytes of crustaceans from Zagreb surroundings was determined. Our results have shown that these freshwater organisms (except snail L. stagnalis) are very suitable for genotoxicity testing by micronucleus test. According to our results, D. polymorpha proved to be the most suitable organism for the assessment of genetic damage. Apart from the laboratory, where MN assay is used for detection of genotoxicity of chemicals, drinking water etc., it can be also used as a field procedure for rapid screening of genotoxic compounds in polluted waters revealing their effect on living organisms.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA