Naslov
Spectrofluorimetric, spectrophotometric and chemometric analysis of wheat grains infested by Sitophilus granarius

Autori
Strelec, Ivica ; Kučko, Lucija ; Roknić, Dragica ; Mrša, Vladimir ; Ugarčić-Hardi, Žaneta

Izvornik
Journal of stored products research (0022-474X) 50 (2012); 42-48

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
wheat quality control; granary weevil infestation; fluorescence; polyphenols; reactive amino groups; UV/VIS spectra

Sažetak
Invisible infestation of wheat grains by Sitophilus granarius during postharvest handling is one of the main problems particularly for small enterprises and farm grain storage facilities due to the lack of cheap and fast methods for early detection of infestation, especially when only a small proportion of grain is infested by larvae. In order to determine whether the spectrofluorimetric, spectrophotometric or chemometric analysis of various extracts of wheat grains infested by S. granarius larvae could be used for detection of infested grain, four different types of extracts: 50 mmol dm
3 Tris-HCl pH 8.9, aqueous isopropanol(4 ¼ 0.55), methanol, and aqueous trichloroacetic acid (g ¼ 0.2 mg cm
3), of non-infested and infested wheat grains have been examined for fluorescence properties and UV/VIS spectral characteristics, while the chemometric analysis included determination of protein, reactive amino groups, polyphenols and soluble sugar content. Analysis of extracts revealed significant differences in UV/VIS spectral characteristics, level of reactive amino groups and polyphenol content between infested and noninfested grains. The results obtained indicate that instrumental analysis of spectral characteristics of grain extracts could be used as a method for determination of grain infestation.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA