Naslov
Comparison of arterial whole blood metabolite determinations in Stat Profile Critical Care Xpress Analyzer with standard laboratory methods

Autori
Perkov, Sonja ; Flegar-Meštrić, Zlata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
16th IFCC – FESCC European Congress of Clinical Biochemistry and Laboratory Medicine – EUROMEDLAB 2005 ; Glasgow. Abstracts. Clin Chim Acta 2005 ; 355, Supplement:S 390, VP12.05. / - : Elsevier, 2005

Skup
16th IFCC – FESCC European Congress of Clinical Biochemistry and Laboratory Medicine – EUROMEDLAB 2005

Mjesto i datum
Glasgow, Ujedinjeno Kraljevstvo, 2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
analytical quality specification; point-of -care testing; biosensor-based analysis; matrix effect.

Sažetak
The rapid progress in technological development in point-of-care testing make it possible to measure a number of analytes in the whole-blood samples. The aim of the present study was the evaluation of biosensor-based methods for the measurement of electrolytes and substrates in the whole blood using Stat Profile Critical Care Xpress multiprofile analyzer and their comparability with standard laboratory methods. Because of the increased utilization of arterial blood samples in hospitalized patients, and a limited information on differences between arterial and venous blood for most routine laboratory tests, analytical differences caused by a different sample type were evaluated. Whole- blood arterial samples and venous serum samples were obtained from 70 unselected patients with a variety of diagnoses admitted to intensive care unit. The multiprofile acid base analyzer Stat Profile Critical Care Xpress (STP CCX), (Nova Biomedical Corp, Walthman, Mass, USA) based on biosensor technology was used to obtained whole-blood electrolyte and substrate profiles. For comparison studies, the plasma or serum samples were analysed with the standard laboratory methods using the Olympus AU 600 analyser (Olympus Mishima Co., Ltd., Shizuoka, Japan). The imprecision expressed by the coefficient of variation (CV%) was less than 5, 7% for all analytes at both high and low concentration except for concentration of creatinine where a CV of 13, 8 % in low and 9, 5% in high concentration level was obtained. The inaccuracy of the electrolytes and substrates measurement in the whole blood using STP CCX analyzer met the analytical quality specification required for near patient testing. Statistically significant correlation (p<0, 05) was obtained between standard laboratory methods performed in arterial plasma or venous serum samples on Olympus AU 600 analyzer and direct whole blood measurements on STP CCX point-of-care analyzer for all parameters tested, althought slope and intercept values showed analytical differences in electrolytes measurement. Least squares linear regression (Deming) between standard laboratory methods in arterial plasma (x) and whole blood (y) showed the folowing results: glucose (y=1, 082x-0, 543 mmol/L, r=0, 963), urea (y=1, 254x-0, 613 mmol/L, r=0, 970), creatinine (y=1, 032x+3, 598 µmol/L, r=0, 956), potassium (y=1, 333x-1, 3 mmol/L, r=0, 918), sodium (y=0, 971x+2, 73 mmol/L, r=0, 939) and chloride (y=0, 712x+30, 59 mmol/L, r=0, 968). The results obtained between standard laboratory methods in venous serum (x) and whole blood (y) were as follows: glucose (y=0, 963x+0, 244 mmol/L, r=0, 951), urea (y=1, 214x-0, 686 mmol/L, r=0, 977), creatinine (y=1, 002x+0, 319 µmol/L, r=0, 981), potassium (y=0, 991x+0, 009 mmol/L, r=0, 973), sodium (y=0, 846x+19, 308 mmol/L, r=0, 981) and chloride (y=0, 800x+20, 40 mmol/L, r=0, 953). The STP CCX multiprofile point- of-care analyzer provides rapid and accurate direct whole blood measurement with acceptable performance compared to standard laboratory methods. The obtained whole blood results for electrolytes and substrates were comparable to the standard laboratory methods in arterial plasma or venous serum samples.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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