Naslov
Analysis of expression and function of proliferation-associated nucleolar gene Nol1

Autori
Gajović, Srećko ; Malnar, Tajana ; Kostović-Knežević, Ljiljana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
11th Ljudevit Jurak Symposium on Comparative Pathology / Krušlin, Božo ; Belicza, Mladen - Zagreb : Hrvatska medicinska akademija, 2000

Skup
11th Ljudevit Jurak Symposium on Comparative Pathology

Mjesto i datum
Zagreb, Hrvatska, 09.06.2000. - 10.06.2000

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
gene trap; Nol1; nucleolus; embryo; mouse

Sažetak
Nol1 gene encodes proliferation associated nucleolar protein 1. Nol1 was originally found as a marker of the nucleolus of various malignant tissues: carcinomas of lung, liver, lymph system, gastrointestinal tract and brain. In the breast carcinoma the level of nucleolar protein 1 has a prognostic significance. In addition, nucleolar protein 1 is present in the nucleoli of non-malignant, but rapidly proliferating tissues. Nol1 expression is regulated during cell cycle: the protein synthesis starts in middle G1 phase and peaks in S phase, which suggests its involvment in the increased nucleolar activity associated with the cell proliferation. The aim of our study was to find if nucleolar protein 1 is involved in embryo development. The mouse carrying insertional mutation within Nol1 gene was produced using the gene trap method. The expression pattern analysis was enabled by inserted lacZ gene transcribed in frame with Nol1 gene. LacZ gene product, ß-galactosidase, was visualized by color changes of its substrate X-gal in blue. The expression pattern of Nol1 gene was studied on 8.5-19-day mouse embryos. During the examined stages of development the expression of Nol1 was found in all tissues but not in every cell of embryo. High staining was found in nucleolus, but low staining was present in the nucleus and the cytoplasm as well. The function of nucleolar protein 1 was studied by analyzing the phenotype of the mice homozygous for gene trap mutation. Using the PCR method we demonstrated that homozygous mice were not present among living offspring and among 11.5-day embryos. Therefore we assumed that homozygous phenotype was embryonic lethal. We intend to analyze homozygous embryos on blastocyst stage, where we expect more data to explain why embryos die. The understanding of Nol1 function during embryo development should open the path to understand its role in tumorigenesis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA