Naslov
The activating NK-receptor NKG2D mediates CD8+ memory T cell formation

Autori
Wensveen, Felix M. ; Gulin, Maja ; Jelenĉić, Vedrana ; Polić Bojan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
2011 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Rabac, Hrvatska, 07.10.2011. - 09.10.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
NKG2D; memory CD8 T cells; LCMV; MCMV; OT-1

Sažetak
NKG2D is an activating receptor, which is highly expressed on NK cells and rapidly induced on activated murine CD8 T cells. Its role in promoting the effector function of cytotoxic cells has been well established. In addition to effector cells, NKG2D expression is high on memory CD8 T cells, but its function on this subset is currently unknown. Here we show that NKG2D promotes memory CD8 T cell formation and survival. Under homeostatic conditions, NKG2D-deficient mice display no gross abnormalities in thymic T cell precursors or mature T cell populations in the periphery. Infection with LCMV or mCMV virus does not lead to differences in absolute numbers or in the functionality of effector type cells. However, in a bone marrow chimeric setting, NKG2D-deficiency results in a disadvantage early during the formation of central memory precursor cells. Proliferation of these cells was not affected, as demonstrated by in vivo BrdU incorporation during infection and by CFSE dilution upon restimulation in vitro. Functionality of NKG2D-deficient memory precursors was not impaired, as these cells were capable of eliciting recall responses of a magnitude comparable to that of Wild Type cells after infection with a second pathogen, or after adoptive transfer of mCMV-specific memory cells in a naïve host and infection with mCMV. We do observe that NKG2D-deficient memory cells are less responsive to the survival-promoting cytokine IL-15, whose receptor is closely associated with NKG2D-signaling. In summary, our data suggests that NKG2D promotes memory CD8 T cell formation by enhancing IL-15 mediated survival of precursor cells early after viral infection.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA